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They were diluted with water prior to analysis by LC-MS/MS on a Sciex API 4000 (Warrington, UK) equipped with a Turbo V electrospray source (ESI)

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Results In the comparative evaluation of model performance, we assessed nine combinations of three feature setsFG, MD, and STwith three tree-based classifiers (RF, XGB, and LGBM)

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Each fourth seedling leaf was collected for subsequent experiments

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