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Quantitative PCR assays were performed using 480 SYBR Green Master reagent mix (Roche Life Science, 4707516001, murine probes) or TaqMan assays (human ETC genes) on a real-time fluorescence qPCR instrument (LightCycler 480II, Roche Life Science)
For post-PCR cleanup, DNA clean beads were added in a volume 1.5 times the volume of DNA
Mechanism of ER stress and inflammation for hepatic insulin resistance in obesity
Some studies claim that glutathione is Glutathione is a very strong antioxidant that the body makes and uses every day
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The animals were raised in a specific pathogen-free (SPF) laboratory with ad libitum access to food and water
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