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A practical default is about 28 days in the refrigerator at 2-8 C when mixed with bacteriostatic water and handled cleanly

Furthermore, the activation of the reactive oxygen species (ROS) pathway and increased glutathione levels indicate increased cellular response to oxidative stress, further contributing to cell survival and proliferation

The increased oxidative stress seems to be the second hit in the progression from NAFL to NASH: the increase in ROS and consequently in lipid peroxidation has been associated to hepatocellular damage, inflammation, and eventual fibrosis due to the activation of hepatic stellate cells