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( b ) Same as (a), but S-phase was assessed by BrdU incorporation (n = 3 technical replicates representative from 2 independent experiments)

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Optimization of operation conditions The variable conditions of fluorescent assay may induce the variable degrees of molecular motion and molecular interaction of the testing system 51,52

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In addition to GSH, cysteine metabolism produces reactive sulfur species such as hydrogen polysulfides (H 2 S n ), which can act as nucleophilic and redox-active metabolites scavenging reactive oxygen species and lipid peroxyl radicals, thereby mitigating lipid peroxidation independently of the GPX4GSH axis [77,78]