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In the second scenario, the final yield of your elution is close to 100 mg of GST-fusion protein, so youre feeling confident that you used the right binding buffer and that your protein isnt too big

Ferroptosis is driven by iron-dependent lipid peroxidation, primarily initiated by glutathione (GSH) depletion or inactivation of GSH peroxidase 4 (GPX4)

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doi: 10.3390/molecules25204738 96 YamauchiM.NigauriA.YamamotoK.NakazatoG.KawanoJ.KimuraK

AccessedMarch 24, 2026.