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In the case of redox stress, two molecules of GSH reduce one molecule of hydrogen peroxide in the presence of glutathione peroxidases, generating one molecule of glutathione disulfide (GSSG), an oxidized form of GSH, and two molecules of water [122, 123]

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By analyzing RNA from E

The glutathione assay was selected as the first screen because it had higher signal-to-noise ratio, more rapid throughput (30 min versus 2 hrs for completion of the senescent assay), lower assay kit cost, and a higher shelf-life of reagents in the kit after reconstitution

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Staining was ended by soaking the leaves in 90 % warm ethanol until the decolorization of tissues was apparent, with the exception of the deep-brown-colored polymerization product produced during the reaction of DAB with H 2 O 2 in the leaf cells