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For high-fat diet experiments, mice were fed a 60 kcal% fat high-fat diet (Research Diets, D12451i) following either tamoxifen or AAV-TBG-Cre injection for 2-3 weeks

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Therefore, changes in GI symptoms should also be quantified, rather than simply assessing their presence or absence

Symptoms Symptoms Aside from the appearance, CMN is usually asymptomatic

Effect of pH on H 2 O 2 Production by GSH Quantification of H 2 O 2 production by 10 and 30 mM GSH at its intrinsic pH (5.4 and 3.3, respectively) and at pH 7 0.2 (buffered using NaOH) in 1 PBS was analyzed using a Hydrogen Peroxide Assay Kit (Fluorometric-Near Infrared) (Abcam, Australia) complemented with a fluorescence plate reader (Tecan infinite M1000 pro)