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HPLC testing is the only definitive way to confirm remaining potency, but that's impractical for most individual researchers

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This accumulation of reactive oxygen species (ROS) disrupts meiotic spindle assembly, impairs spindle assembly checkpoint signaling, compromises chromatin cohesion, and ultimately deteriorates oocyte quality and developmental potential in women of advanced reproductive age (17)

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PLoS ONE 11 , e0160264 (2016)