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For the assessment of kidney injury severity (severity score), HE stained section images at 10 and 20 (2 at each magnification, for a total of 4 images per mouse) were graded from 0 (no damage) to 5 (most severe damage observed across the samples analyzed), based on the following parameters: (i) protein cast formation, (ii) visible tubular dilation, (iii) loss of proximal tubular cell polarization (no visible brush-border membranes) (iv) area affected (with single nephron damage to majority of the cortex with visual signs of injury)

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GLP-1 medications must only be injected subcutaneously and never into a vein

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The original immunohistochemistry slides were scanned by Aperio Versa (Leica Biosystems) which captured digital images of the immunostained slides

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