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For example, during ferroptosis, mitochondria condense and become small, and the lipid bilayer membrane thickens and contracts with the outer membrane rupture

ATP, adenosine triphosphate

Cells were washed twice in PBS containing 10% FCS and finally digested in Hanks Balanced Salt Solution (HBSS) containing 100 mg Dispase II/100 ml under repeated shaking for 10 min at 37 C