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After TMB substrate solution is added, only those wells that contain Rabbit TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

Specifically, SIRT3 deacetylates and inhibits aldehyde dehydrogenase 2, which prevents the conversion of acetaldehyde to acetate, potentially leading to acetaldehyde toxicity 183

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The public health impact and cost-effectiveness of the R21/Matrix-M malaria vaccine: a mathematical modelling study