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Proteomic analysis Cells were seeded in 10-cm dishes and cultured for 7 days in cystine-free medium supplemented with heavy isotope-labelled 13 C 6 , 15 N 2 -cystine (Cambridge Isotope Laboratories, CNLM-4244-H)

In all cases, it is crucial when the MRI was performed, not only because degenerative changes may take time to evolve, but also because acute traumatic disc alteration, such as bleeding into the disc compartment or disc oedema, may mask other structural or degenerative changes

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