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The extract was rotary evaporated at 30 C to a volume of 2 ml, acidified to pH 4 with formic acid, and 200 l aliquots were purified using preparative HPLC (Agilent Technologies, 1260 Infinity) over a Luna 5 m phenyl-hexyl 100 (250 10 mm) column and a 15 min linear gradient from 0% acetonitrile in H 2 O to 16% acetonitrile in H 2 O at 4 ml min 1 flow
Look for easy-to-tolerate combinations of carbs and proteinyogurt with fruit, half a banana with peanut butter, or a handful of nuts
10.1021/acs.jafc.1c04035 128
Vitamin B12 is an essential nutrient that supports nerve health, red blood cell production, and DNA synthesis
Plus, it helps with fat breakdown and energy creation, boosting our overall health