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Both endothelial cells and aortic sections were treated with 0.5% (v/v) Triton X-100 for 15 min, followed by three washes with PBS, each time for 5 min, and then blocked with 5% (v/v) bovine serum albumin (BSA, Sigma-Aldrich, B2064) at room temperature for 1 h

These findings unveil a corticothalamic circuit that bidirectionally regulates pain processing and underlies fear-provoked analgesia, offering potential therapeutic avenues for pain management

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1998 Sep 5;352(9130):763-6

Part of the variability might be due to the different analytical methods and the differences in the processing and/or storage of the biological samples