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After 2 months, the animals were subjected to X-ray and MRI

Contrast-enhanced ultrasound is a more recent technique that involves the injection of microbubbles intravenously prior to imaging, which enhances the ultrasound signals and allows for more detailed measurements of blood flow

This stepped approach allows the body to develop tolerance to GLP-1 effects, minimizing severe side effects and preventing accidental overdosing

After heat-inactivating the samples (70 C, 5 min), each sample was divided in two aliquots, and 3 l of Porcine kidney trehalase (Sigma) was added to one of the aliquots to break down trehalose into glucose

Age-specific timeline example (38-year-old woman): A compressed approach might look like this: spend months 0-4 on GLP-1 treatment, then months 4-6 in the washout period (2 months), and by month 6 begin trying or proceed to IVF