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Instead of using animal cells, they took skin cells from elderly human donors and genetically transformed them directly into brain cells (neurons)

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Cell Culture and GSH Preparation K562, HepG2, Hela and L-02 cells were respectively cultured in RPMI 1640 (Hyclone, penicillin 100 U/mL, streptomycin 100 g/mL) adding 10% fetal bovine serum and maintained at 37 C in a humidified atmosphere including 5% CO 2 , in terms of the instructions of the American Type Culture Collection

First, SPI1 (also known as PU.1) is a transcription factor crucial for microglia viability and differentiation, located within a genome-wide significant AD-risk locus, where reduced expression is associated with delayed AD onset

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