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IBRO Neurosci Rep 15:225234

Sumida Y, Yoneda M (2018) Current and future pharmacological therapies for NAFLD/NASH

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For H 2 O 2 assessment in isolated mitochondria, 50 g protein-aliquots of freshly obtained mitochondria were incubated with 100 M AmplexRed probe (Invitrogen) in mitochondrial respiration buffer (125 mM KCl, 2 mM KH 2 PO 4 , 1 mM MgCl 2 , 0.5 mg ml 1 bovine serum albumin and 10 mM HEPES, pH 7.4) containing horseradish peroxidase (0.5 U ml 1 )

GLP-1 medications work by mimicking a naturally occurring hormone called glucagon-like peptide-1, which helps regulate blood sugar and appetite