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Interleukin-1beta contributes via nitric oxide to the upregulation and functional activity of the zinc transporter Zip14 (Slc39a14) in murine hepatocytes

For protein analysis, cell pellets were resuspended in 1.5x protein sample buffer (PSB) (50 ml of 4.5 x PSB containing 15 ml glycerol, 7.5 ml -mercaptoethanol, 22.5 ml sodium dodecyl sulfate [20%], 6.3 ml Tris buffer, pH = 6.8 [1M], and bromophenol blue)

Always record any reactions observed during testing

The dose is adjusted based on severity and response to treatment

According to the regulations of the International Civil Aviation Organization (ICAO) on preventing explosive liquids from being brought onto a plane, liquids and gel-type products are permitted onboard in limited quantities only

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