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Higher strengths can cause redness and stinging

Cells at 20 ~ 30% confluency were transfected with 3 g pcDNA3.3-dsRed/Sabin1-P1 or pcDNA3.3-dsRed/Mahoney-P1 formulated with Lipo3000 transfection reagent (Invitrogen), and 2 g pcDNA3.3-T7 and 2 g pT7-Replicon-eGFP were co-transfected the next day

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