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Dermatol Pract Concept 8, 1521.CrossRefGoogle ScholarPubMed Kim, KY, Lee, EJ, Whang, WK, et al

For transfection of Lenti-X 293T, cells were seeded in the 6-well plate on the first day and transfected on the second day when the cells reached approximately 70%90% confluent, as described previously ( Confocal fluorescence microscopy analysis Lenti-X 293T cells were seeded on cell slides in 24 well-plate on the first day and transfected on the next day when cells reached 30%50% confluence

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This indicated that the increased CYSS levels might originate from auto-oxidation of CYS, which easily takes place out of the cell under normoxic conditions (43, 44)

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Here, too, no clinical effects on tumor growth were achieved with the KD as a monotherapy, with a median progression-free survival of 5 weeks (range 313 weeks)