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Reverse transcription and quantitative real-time PCR (qPCR) Total RNA was extracted from frozen fundus and antrum mucosal scrapings from lean or HFD obese rats with TRIzol reagent (Invitrogen, Saint Aubin, France)

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The mechanisms are completely separate

Both diarrhea (33.3% at 12 mg) and constipation (16.7% at 12 mg) were reported in Phase 2, and both can produce lower abdominal cramping through different mechanisms researchers sometimes describe this presentation as retatrutide gas pain or trapped-gas-style pressure rather than classic stomach cramps

In the final months of the Biden administration, the FDA convened the expert advisory committee and presented its research on the six peptides