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The following day medium was removed and replaced with 20 l of loading medium consisting of 1:1 (v/v) Opti-MEM: Hanks balanced salt solution (HBSS), 2.5% (v/v) FBS, 20 mM HEPES, pH 7.4, 2.5 mM probenicid and fluorescent indicator 2 M Fluo-4 AM (Invitrogen)

Mass Spectrometry for Peptide Analysis ESI-MS and MALDI-TOF methods for confirming peptide identity

N., Alam, A

The visual appearance of the solution does not indicate whether mechanical denaturation occurred

The right dosage for you depends on several factors, and its crucial to work with a qualified healthcare provider who can assess your individual needs and medical history