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Stock solutions of test compounds that are water soluble were made up at 20 times the final assay concentration in buffer (including glucose, GEP44, GEP12, exendin-4, PYY 1-36 and insulin)

Resistin promotes CCL4 expression through toll-like receptor-4 and activation of the p38-MAPK and NF-B signaling pathways: implications for intervertebral disc degeneration

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Briefly, approximately 3040 mg of frozen kidney was homogenized in 2 volumes of Qiazol reagent (Qiagen GmbH, Hilden, Germany) and then centrifuged at 10,000 g for 15 min at 4C