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The samples were subsequently reduced with 4 mM DTT for 30 min at room temperature with shaking at 1,000 rpm, followed by alkylation with 10 mM iodoacetamide for 45 min in the dark at room temperature while shaking at 1,000 rpm

This suggests that a significant portion of follicular loss in transplanted ovarian tissue may be attributed to the extensive apoptosis of granulosa and stromal cells (66)

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The authors pointed out that if even small amounts of D-Carnitine posed a threat to the normal functioning of the bodys metabolism, then arguably accurate analytical methods would be critical in the pharmaceutical and clinical use of the product

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