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Individual absorption : Factors like age and digestive health can affect B12 absorption

The absorbance (490 nm) was measured three hours after incubation using a microplate reader (Tecan)

But theres another catch, because cysteine cannot be given as such, we had to supplement it in another form called N-acetylcysteine. In past studies, Sekhar and his colleagues determined that supplementing GlyNAC, a combination of glycine and N-acetylcysteine, corrected glutathione deficiency inside the cells of naturally aged mice to the levels found in younger mice

Spontaneous hydrolysis and spurious metabolic properties of -ketoglutarate esters

Doctors can diagnose vitamin B12 deficiency can be diagnosed through blood tests, including a complete blood count and methylmalonic acid test, to measure B12 levels and metabolic function

10.1089/jop.2009.0023 J Ocul Pharmacol Ther