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X-tremeGENE HP DNA Transfection Reagent (Roche) was used for transient transfection, and the total amount of transfected DNA was normalized by addition of empty control plasmids

The FDA findings regarding inadequate unidirectional airflow in ISO 5 areas and equipment surfaces that were difficult to clean or visibly dirty suggest USP 797 compliance gaps during the inspection period

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Harlow SD, Gass M, Hall JE, et al

doi: 10.1007/s00125-011-2409-9 72 ZhangJZhongHBLinYYaoWHuangJY