Labelled cells were then detached by trypsinization, pelleted, washed once with PBS, resuspended in 300 l ice-cold PBS and fixed by adding 700 l ice-cold absolute ethanol while gently vortexing
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doi: 10.1080/15476286.2020.1737787 7 ChenCJChinJEUedaKClarkDPPastanIGottesmanMMet al
Based on the here-presented biochemical, structural and computational evidence, we propose that arGSTs utilize a GSH-dependent Brnsted base mechanism for conversion of the semi-stable LDOX product flavan-3,3,4-triol ( 4 ) or flavan-3-on-4-ol ( 7 ) into flav-2-en-3,4-diol ( 8 -B4), the 4-hydration species of cyanidin ( 8 ) (Extended Data Fig
The synthesis of PC 5 was also attributed to the presence of Arsenic stress and SiNPs