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To study the intracellular levels of ROS and GSH in viable cells, the samples were incubated with 10 M CellTracker Blue (Thermo Fisher Scientific, Barcelona, Spain) and 10 M CM-H 2 DCFDA (Thermo Fisher Scientific, Barcelona, Spain) for 30 min at 38.5 C, followed by 0.5 M PI staining

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doi: 10.1097/PRS.0b013e31828275b7

Collectively, GGCT emerges as a pivotal metabolic orchestrator in HCC and PCa, functioning as a bidirectional switch that diverts Gln toward GSH biosynthesis for redox defense while enhancing Glc-fueled TCA anaplerosis to sustain cell proliferation

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typical viscosity around 40,000120,000 cps