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Then, absorbance values at OD 412 nm were continuously recorded every 2 min for 10 min at 25 C

These biochemical changes are accompanied by ultrastructural abnormalities with the presence of a lesser number of mitochondria that appear bloated and round, with the presence of paracrystalline inclusions and loss of cristae [90,91]

Splenocytes were washed with warm RPMI 1640, stained at 37 C for 30 min with Abs diluted in RPMI 1640, and washed with PBS

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Injectable GHK-Cu protocol: Dose: 1 to 2 mg per injection

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