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Furthermore, Holt et al, 138 intra-peritoneally injected exendin-4 to the spinal cord of rats and detected an increase HR although this did not influence the arterial blood pressure, which is independent of PPG neurons activation in NTS, and can be blocked by a -adrenoceptor antagonist

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Briefly, lesion DNA was isolated using the QIAmp DNA Mini kit (QIAGEN) following the manufacturers instructions