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Membranes were probed using antibodies to FoxO1 (1:2500, 18592-1AP, Proteintech, Chicago, IL, USA), SOD2 (1:6000, 66474-1lg, Proteintech, Chicago, IL, USA), NOX4 (1:600, 14347-1-AP, Proteintech, Chicago, IL, USA), and -actin (1:2000, AM10218-400, Abgent) as the primary antibodies

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Higher-risk groups include older adults, those following vegetarian or vegan diets, patients taking metformin or long-term proton pump inhibitors, and individuals with a history of gastrointestinal surgery or conditions such as pernicious anaemia or coeliac disease

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