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Vasorelaxation at least 60% by 3 M ACh in rings pre-contracted with 3 M PE was considered endothelium intact

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Heterogeneity in dosing (4001500 mg daily) and formulation quality complicates direct comparison across trials

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These properties have established it as a widely used reference compound in studies examining melanocortin receptor signaling, receptor selectivity, ligand-receptor interactions, and comparative pharmacology among melanocortin receptor agonists and related peptide analogues.

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