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Enzymatic antioxidants are localized in specific subcellular compartments where radicals are frequently generated (mitochondria, peroxisomes or microbodies, cell cytosol) or are present in the systemic circulation

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It disrupts the pattern the protocol is designed around

However, the extent to which SLC7A11 dependency defines ferroptosis sensitivity varies substantially across cell types, metabolic states, and microenvironmental nutrient availability

The aim is to raise NAD+ levels quickly so it can enter cells and get to work