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The reaction mixtures were then centrifuged for 5 min at 6000 g and the supernatants assayed for DNA oxidation with an 8-hydroxy-2-deoxyguanosine (8-OHdG) ELISA assay (Cell Biolabs, CA, USA)

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In this stage of regeneration, three groups of enzymes are important: glutathione oxidase (GO) and glutathione peroxidase (GSH-Px), which catalyze the oxidation of GSH to GSSG, and the enzyme glutathione reductase (GR) that is responsible for the regeneration of GSH, from GSSG, in the presence of NADPH [33]