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When adding the water to the peptide vial, do not aim directly at the powder

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Note: If you currently receive B12 injections for a diagnosed medical condition, consult your healthcare provider before making any changes to your treatment plan.

Similar to ORD, the shape of the CD spectrum in the peptide region (240 nm and below) is determined by the contributions of different secondary structure elements (with -helices, -sheets, -turns, and unordered/random coil being the most frequently considered structural motifs) and can therefore be used for the estimation of the secondary structure composition of a protein (Kelly et al., 2005

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