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Since the inner mitochondrial membrane is impermeable to acyl-CoA on its own, fatty acyl-CoA on the outer mitochondrial membrane binds to L-carnitine through the action of carnitine acyl transferase I enzyme to temporarily generate fatty acyl carnitine

Its usually administered intramuscularly or subcutaneously using sterile technique

[5] Orer GE, Guzel NA

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L-Carnitine is made by the body mostly in the liver and kidneys where the amino acids lysine and methionine are used