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doi: 10.3389/fendo.2019.00482 74 De LeoSLeeSYBravermanLE

Changing BAC water volume changes the concentration (mg/mL), which changes the draw volume for the same mg dose

By mimicking natural copper-binding sequences, it supports detailed exploration of collagen synthesis, endothelial signaling, and antioxidant enzyme activationwithout engaging systemic hormonal pathways

Preparation of NEM-derivatized cysteine and GSH internal standards The N-ethylmaleimide (NEM) derivatized isotope labeled [ 13 C 3 , 15 N]-cysteine-NEM and [ 13 C 2 , 15 N]-GSH-NEM were prepared by derivatizing the [ 13 C 3 , 15N]-cysteine and [ 13 C 2 , 15 N]-GSH standards with 50 mM NEM in 10 mM ammonium formate (pH = 7.0) at room temperature (30 min) as previously described 95

This detoxification function is particularly vital in the liver, where glutathione is abundant