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Adherent cells were scrapped, then centrifuged at 4C and 13000 g for 15 min, and the resulting supernatants processed using established protocols.[19] Intracellular levels of 1-MNA and as well as the IS were determined from LC/MS/MS peak areas

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Methionine is needed to make creatine, a nutrient naturally found primarily in muscle tissue that provides the energy our muscles need to move, and has been found to boost athletic performance during short, intense workouts

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