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Surviving death: emerging concepts of RIPK3 and MLKL ubiquitination in the regulation of necroptosis

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Cell death and inflammation were induced in OCTN2-deficient, but not wild-type, tubular cells exposed to high salt and high glucose

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The researchers examined the impact of these interventions through a biochemical characterization of brain homogenates, quantifying oxidative and inflammatory markers such as lipid peroxides, glutathione (GSH), glutathione S-transferase (GST), 5-lipoxygenase (5-LOX), cyclooxygenase-2 (COX-2), leukotriene B4, prostaglandin E2, and ascorbic acid using spectrophotometric assays and ELISA