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MAPK pathway involved in epidermal terminal differentiation of normal human epidermal keratinocytes

Immunofluorescence assay SK-MEL-147 cells were collected and seeded into the inner 60 wells of 96-well, black-walled plates (Agilent, 204626-100) at 1000 cells per well in 100 uL of complete DMEM overnight in a 37 C incubator with 5% CO 2

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Such speculation is therefore not a basis for clinical concern at this stage