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Left ventricular cardiac tissue was cut into small pieces approximately 2-3 mm thick, immediately fixed in electron microscopic solution (2.5% glutaraldehyde) for 24 hours, stained with toluidine blue for the more conspicuous areas, stained with 4% methanolic uranyl acetate and Reynolds lead citrate stain, and observed with a transmission electron microscope to observe the ultrastructure of mouse myocardial mitochondria (TM -3000