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Antibiotic medium (MEM, 2% PS, 12.5 g/mL amphotericin B, 0.5 mg/mL gentamicin sulfate) was added and the cells were cultured in an incubator (28C + 5% CO2) for 2 hours, with a medium change every 30 minutes

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Author contributions JW designed the research, JW and RWcontributed to the literature database search, data collection, data extraction, data analysis, and writing of the manuscript