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Zeng Z, Yang D, Huang X, Xiao Z

they are also unresponsive to recall antigens and unable to secrete normal amounts of interleukin-2, exhibiting properties similar to a state of anergy

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Measurement of intracellular ROS and GSH levels MII-stage oocytes and 4-cell stage embryos were cultured with 10 M 2,7-dichlorodihydrofluorescein diacetate (DCFH, #S0033, Beyotime, Shanghai, China) or 10 M 4-chloromethyl-6,8-difluoro-7-hydroxycoumarin (CMF2HC, #C12881, Invitrogen,) at 37C for 45 min and 30 min in darkness, respectively