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In addition, cancer cells may undergo metabolic reprogramming to adapt to cholesterol depletion

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Cells were harvested and centrifuged at 1200 rpm for 3 min, followed by incubation with 1 L annexin V-Alexa Fluor 488 (640906, BioLegend) in 100 L annexin V binding buffer 1 (10 mM HEPES (pH 7.4), 0.14 M sodium chloride (NaCl), 2.5 mM calcium chloride (CaCl 2 ) and incubated at room temperature in the dark for 15 min

The size of aggregations is measured by comparing it with red blood cells

Chest Pain (Angina) Plaque buildup in the coronary arteries can limit blood flow to the heart, triggering chest discomfort during physical or emotional stress

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