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Chlorogenic acid ameliorates CCl(4)-induced liver fibrosis by modulating the PI3K/AKT/mTOR autophagy pathway

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The pooled library was then enriched with an additional 6 PCR cycles using P5 and P7 primers and gel-purified from a 1% agarose gel (Seakem) using the QiaQuick gel extraction kit (Qiagen)

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Therefore, Schiff-base (SB) reactions between DAQs and peptides can be excluded