10.1055/a-1792-2678 86 RubioloJ
Rome wasnt built in a day
Lipids were separated at a flow rate of 0.3 mL/min and a column temperature of 50C by a gradient elution starting at 10% B increasing in 20 min to 80% B, followed by an increase to 95% B within 4 min, and further 3 min until 100% B is reached and kept for 5 min, followed by a decrease to 10%B within 0.1 min and equilibration at 10% B for 7.9 min 56
Brains were homogenized using lysis buffer for whole protein and mitochondrial protein extraction as previously described (Nguyen et al., Novex 4%12% Bis-Tris gels (Life Technologies, Carlsbad, CA, USA), then transferred to PVDF membranes (Rockford, IL, USA), which were blocked with non-fat milk and incubated with primary antibodies (OXPHOS complexes
skin, recovery, growth hormone release)
A.CoppolaG.TsukadaS.SchroederB