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To further explore the underlying mechanism of GSH in activating systemic plant defense responses, we introduced the [Ca 2+ ] cyt reporter GCaMP3 into pad2-1 , generating GCaMP3 / pad2-1 plant, to investigate whether the GSH-deficient mutant attenuated the wound-induced systemic [Ca 2+ ] cyt transmission

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Thermo Fisher Scientific, Waltham, MA, USA) and -MEM (Thermo Fisher Scientific), respectively, supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (P/S) at 37 C and 5% CO

It is also leveraging connected devices and mobile apps to support adherence and remote monitoring

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