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In the second study 36 patients with OSA and T2D were divided into 2 arms: (i) DAPA arm ( n = 18) all patients received DAPA in a daily dose of 5 mg, which was increased to 10 mg 1 week later

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Metabolite analysis was performed by liquid chromatography-mass spectrometry LC-MS using hydrophilic interaction liquid chromatography (HILIC) and high-resolution (Q-Exactive Orbitrap, Thermo Fisher) MS as previously described 32, 38

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