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In addition to the complexity of the PDHc at the level of the total number of protein subunits (30 subunits of E1, 60 subunits of E2, and 12 subunits of E3), the full activity of the PDHc requires five different vitamin-derived coenzymes: CoASH, NAD + , FAD, lipoic acid, and thiamine pyrophosphate (TPP)

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The amplitude of the fluctuations and membrane instabilities increased over time over a typical time course of 10 seconds (Fig

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