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At the end point, cells were rapidly washed with ice-cold PBS and incubated at 80 C for 30 min with extraction solvent at a ratio of 1 ml per 1 10 6 cells

Pathologie Biologie (2010) 58:296300

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Soluble amyloid beta-protein dimers isolated from Alzheimer cortex directly induce Tau hyperphosphorylation and neuritic degeneration